DNA

Part:BBa_K1356000:Design

Designed by: Jordan Berg   Group: iGEM14_BYU_Provo   (2014-10-06)

Alpha Amylase with TolB Signaling Sequence and PstI Site Removed


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DsbA signaling sequence was synthesized using RNA primers overlap-extension PCR owing it the signaling sequence's large size. The mutation was done through mutagenic PCR.

Source

The original amylase we modified was from part BBa_K1195001 in the iGem parts registry.

References

1. Kalpana, Balu, Subramonian Aarthy, and Shunmugiah Pandian. "Antibiofilm Activity of α-Amylase from Bacillus Subtilis S8-18 Against Biofilm Forming Human Bacterial Pathogens." Applied Biochemistry and Biotechnology 167.6 (2012): 1778-94. Print. 2. Harrison, Clarice. Part:BBa_K1195001. Aug 28 2013.Web. <https://parts.igem.org/Part:BBa_K1195001>.